Insertion junction: CLIP2.052599_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.052599
Chromosome:chromosome 11
Location:1195667
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g467686 (1 of 51) PF05548 - Gametolysin peptidase M11 (Peptidase_M11) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACCAGTCACTACTGTGTGCGCCCATGTAGACACGTATTAAAAAAACACG
Internal bar code:AGATCTGCCTACTGTTGATGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1011
LEAP-Seq percent confirming:77.7778
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:CACACACACGCCTGTCTTTG
Suggested primer 2:GGAGCACCTCGAGATGTACG