Insertion junction: CLIP2.052628_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.052628
Chromosome:chromosome 12
Location:6358550
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g537100 TSV,TSV2 Valyl-tRNA synthetase; (1 of 1) PTHR11946//PTHR11946:SF5 - ISOLEUCYL, LEUCYL, TYROSYL, VALYL AND METHIONYL-TRNA SYNTHETASES // VALYL-TRNA SYNTHETASE, ISOFORM A intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTGCCTACCGCCCCTGCGGTTCGTCCTCCCAGCCGCCTGCGTGCTCACC
Internal bar code:TGGTAAGATGTGGGGTAAAATC

Confirmation - LEAP-Seq

LEAP-Seq distance:1089
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GATTGGTGTGTGTGTGTGGC
Suggested primer 2:ACACTCCTCGTCACCACAAC