| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.052643 |
| Chromosome: | chromosome 10 |
| Location: | 4983393 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g454300 | (1 of 1) IPR003613//IPR011009 - U box domain // Protein kinase-like domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCGTCTCCTTTTGTGCTTTGCCTGACATAGGCCTCTCGATGCGGCGCGA |
| Internal bar code: | CCTAGGGCAGATTGCTTGTGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2207 |
| LEAP-Seq percent confirming: | 18.9189 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACCCGAAACGTACAGTCGT |
| Suggested primer 2: | GGACGGTCAATTGACGCAAC |