| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.052647 |
| Chromosome: | chromosome 17 |
| Location: | 4339689 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g730300 | (1 of 58) PTHR12393//PTHR12393:SF6 - SPHINGOMYELIN PHOSPHODIESTERASE RELATED // SPHINGOMYELIN PHOSPHODIESTERASE 2 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGAGCCCCTGGCAGCGGCGGTGGCTGGCGGATCGCTACCTGGGGGCTG |
| Internal bar code: | AGGTTTCTAGGTCTCTCTGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1257 |
| LEAP-Seq percent confirming: | 40.7407 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTTGATGGGCGTTACCGA |
| Suggested primer 2: | GGTAACACAGCGACCCTCAA |