| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.052652 |
| Chromosome: | chromosome 17 |
| Location: | 6861249 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g747397 | (1 of 1) PTHR24362//PTHR24362:SF284 - SERINE/THREONINE-PROTEIN KINASE NEK // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCAGGGCCCGGAACACGTAGGCCTTGAGACTGAGCACCTCACGGCTGGA |
| Internal bar code: | CCTGTCGCAGGACGGTCGGGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 400 |
| LEAP-Seq percent confirming: | 50.0 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGAGATGTACCACGTGAGG |
| Suggested primer 2: | CAACGCATATGGCACCATGG |