| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.052653 |
| Chromosome: | chromosome 7 |
| Location: | 1448663 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g323650 | (1 of 1) PTHR13109//PTHR13109:SF7 - NEUROCHONDRIN // NEUROCHONDRIN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCTGTGTTAAAGAGCACAAGGAAAATATAGCGCAAAGACAGTGTATGCG |
| Internal bar code: | GCATTATTCCCCTTCTCCGATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4556 |
| LEAP-Seq percent confirming: | 87.2727 |
| LEAP-Seq n confirming: | 48 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 55 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGTGTTGCCTTGTGTGAG |
| Suggested primer 2: | CCACTCTTGCACAAACCTGC |