| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.052665 |
| Chromosome: | chromosome 12 |
| Location: | 9019596 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g544900 | (1 of 1) PTHR16470:SF0 - UBIQUITIN DOMAIN-CONTAINING PROTEIN UBFD1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGGGCGTGCAGAGCCTGATCTGATATGATATGATCTGACAGTTTACTG |
| Internal bar code: | ACAGTGTCCCACTGTGATCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 279 |
| LEAP-Seq percent confirming: | 13.3333 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 52 |
| LEAP-Seq n unique pos: | 60 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCCCACAAACCACTGTCAA |
| Suggested primer 2: | GCAGATCTGGATCGGGTCTG |