Insertion junction: CLIP2.052667_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.052667
Chromosome:chromosome 11
Location:3419996
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g477200 IFR1 Putative 2'-hydroxyisoflavone reductase; (1 of 19) PF01370 - NAD dependent epimerase/dehydratase family (Epimerase) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACGGCAGGCAATCTTAGATGAGGGGGCGGGATGGGGACGGGGATGGCGC
Internal bar code:GATGTTGTTAGGGTGATGAAC

Confirmation - LEAP-Seq

LEAP-Seq distance:322
LEAP-Seq percent confirming:77.7778
LEAP-Seq n confirming:14
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:CAATCAGCGCATGCATCACA
Suggested primer 2:GTGCTGTTTGTGAGCTGGTG