| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.052688 |
| Chromosome: | chromosome 9 |
| Location: | 5654287 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g410150 | (1 of 140) IPR002048 - EF-hand domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTTGAACGTGAGGCCTAAGTTCACAGTGGGTTTCCAGGCATGTCATGGC |
| Internal bar code: | TGTAAAATCTCCTATGAGTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1068 |
| LEAP-Seq percent confirming: | 92.0 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGTAGTCCCGATTTGCGCA |
| Suggested primer 2: | GATCGCAGCAGCATCTTGTG |