Insertion junction: CLIP2.052694_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.052694
Chromosome:chromosome 5
Location:3083221
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre24.g755597 Chlorophyll a /Pheophorbide a oxygenase-related protein; (1 of 2) 1.14.13.122 - Chlorophyllide-a oxygenase / Cholorophyll-b synthase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGCATGCTTCAACCTTGCCAACACACCAGGAAACCCATCATTTTCCAAC
Internal bar code:CCCAACAGGTGTTGACCAGTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:630
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:CCCCTTGACACACCAGATCC
Suggested primer 2:CCACCGCCTTCATCAGACAT