Insertion junction: CLIP2.052695_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.052695
Chromosome:chromosome 2
Location:7784679
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g146550 (1 of 1) PF14251 - Domain of unknown function (DUF4346) (DUF4346) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCACGAGGTGGTGGTCATAACCCGTCGTCACGACCATCGCTTGCAATGCG
Internal bar code:GACAATTTGGCTTACGCCTTAA

Confirmation - LEAP-Seq

LEAP-Seq distance:2344
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:33
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:33

Suggested primers for confirmation by PCR

Suggested primer 1:TTGATGATGAGACGGGCGAG
Suggested primer 2:ACACACACGTGACCCATCTC