Insertion junction: CLIP2.052696_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.052696
Chromosome:chromosome 3
Location:1325967
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g150450 (1 of 11) IPR023296 - Glycosyl hydrolase, five-bladed beta-propellor domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCCACCTGTAGAAGAAGAAGCTGTAGTCGACGTGGTAGTGCTTATACAC
Internal bar code:GTTCGGTAGGCAATTGCTAAAT

Confirmation - LEAP-Seq

LEAP-Seq distance:2326
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:13
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:TCAAGGTCATCGGCTATCGC
Suggested primer 2:ACCAGGTGCTCCATCATGTG