Insertion junction: CLIP2.052697_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.052697
Chromosome:chromosome 13
Location:1217466
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g570200 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCCGTTGTACCCCCCTCCCTCCCTTCCAAACCCAGCCTTGGCGCAGCGA
Internal bar code:CAGGCAAACGTGTTTAGACATG

Confirmation - LEAP-Seq

LEAP-Seq distance:778
LEAP-Seq percent confirming:90.0
LEAP-Seq n confirming:18
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:CGAATAGCGGTTGCCACATG
Suggested primer 2:GACGCTCACCAAACTGCAAG