| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.052710 |
| Chromosome: | chromosome 1 |
| Location: | 1555477 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g800029 | (1 of 55) PTHR23257//PTHR23257:SF394 - SERINE-THREONINE PROTEIN KINASE // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGATCTACACCAGAAACACATACACAACCCGCTCGCTCGCCCGCACGCA |
| Internal bar code: | GACTTTCCGAGATTGGCACGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4138 |
| LEAP-Seq percent confirming: | 97.8947 |
| LEAP-Seq n confirming: | 93 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 95 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAATCCACCCACCCTCGAAA |
| Suggested primer 2: | AGCAAGATGAGGGAAAGGGC |