| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.052715 |
| Chromosome: | chromosome 7 |
| Location: | 615693 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g316850 | MCM4 | (1 of 1) K02212 - DNA replication licensing factor MCM4 (MCM4, CDC54); Minichromosome maintenance protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTCCACGTTTGGCCTGGAGCGTTTGGCCTGACCTGCGTACCCCTTCCCC |
| Internal bar code: | GTGATGGGTAGGGTTAGTAGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 447 |
| LEAP-Seq percent confirming: | 50.0 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGCTGGGAAAAGAGAGCA |
| Suggested primer 2: | GTTTTCGGTGTTCCACGAGC |