| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.052748 |
| Chromosome: | chromosome 11 |
| Location: | 2315590 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g095137 | PFR1 | (1 of 1) 1.2.7.1 - Pyruvate synthase / Pyruvic-ferredoxin oxidoreductase; Pyruvate ferredoxin oxidoreductase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGCCCTGGCGACAGATCTTGCCCACAGCGGGAGCTTTGAGGTGGGCCT |
| Internal bar code: | CCAGAGGAAGGCCTTTTTTTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1752 |
| LEAP-Seq percent confirming: | 94.7368 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGACCAGTTACGTGCACCAG |
| Suggested primer 2: | GATGTGATGTGATGTGCGCC |