Insertion junction: CLIP2.052750_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.052750
Chromosome:chromosome 3
Location:2767506
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g161550 FAP55 (1 of 1) IPR009053//IPR023220 - Prefoldin // Type IV secretion system, VirB5-domain; Flagellar Associated Protein 55 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTGCGTTGAGGCGGGTGCACTCATCGACATTTCCCTGTAAGACTCCGCC
Internal bar code:TAAATTAGTTACATAATATACT

Confirmation - LEAP-Seq

LEAP-Seq distance:3642
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:15
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:TTGACCTTGTCCTCCATCGC
Suggested primer 2:CGCATAAGCATGACAGTGCC