Insertion junction: CLIP2.052757_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.052757
Chromosome:plastome
Location:21367
Confidence (%):80
Locus systematic id Locus common name Defline Feature
CreCp.g802272 rpl36,2717053,secX,ChreCp010 (1 of 2) PF00444 - Ribosomal protein L36 (Ribosomal_L36); 50S ribosomal protein L36 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTTCATAAAAATTTTATTAGTTTTGAAGTCAATTCGCTTATATGCATCCA
Internal bar code:TATAGTAATAATTGCGACATTT

Confirmation - LEAP-Seq

LEAP-Seq distance:155
LEAP-Seq percent confirming:80.0
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CCACTGCCTGCTTCACCTTA
Suggested primer 2:AGACCCCAACGGCGTTTATT