| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.052794 |
| Chromosome: | chromosome 13 |
| Location: | 2785468 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g801538 | (1 of 73) IPR000477//IPR005135 - Reverse transcriptase domain // Endonuclease/exonuclease/phosphatase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGTCCTAACATATCCCGTCGCGCAGCCGCTAGCTCCTCCGTCCACCAG |
| Internal bar code: | ATAGTTTGGTTTGCAAAAGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4167 |
| LEAP-Seq percent confirming: | 64.3478 |
| LEAP-Seq n confirming: | 74 |
| LEAP-Seq n nonconfirming: | 41 |
| LEAP-Seq n unique pos: | 115 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGAAGGCGAGGACGAGATC |
| Suggested primer 2: | GTCCGAAACGTCACCCTTCT |