Insertion junction: CLIP2.052795_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.052795
Chromosome:chromosome 1
Location:6917710
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g049650 (1 of 32) 3.6.4.4 - Plus-end-directed kinesin ATPase / Kinesin CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGAAGCAGCTGGCGGCTCCAGACGAGTCACTGATGGTGGTCGCGGAAGC
Internal bar code:CTAATCTTCTTTTGATTTTAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:4712
LEAP-Seq percent confirming:94.8718
LEAP-Seq n confirming:37
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:39

Suggested primers for confirmation by PCR

Suggested primer 1:ATCTTCACCCGGCAGAAGTG
Suggested primer 2:GGTCTTCAAACGGAATGCGG