| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.052798 |
| Chromosome: | chromosome 17 |
| Location: | 3668772 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g802071 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGACGAAGGCCAGCATGACAGTTTGGGACATATCCCGCCACGCTGAGC |
| Internal bar code: | GAAGTGCAGTGGGGGGTAATTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 600 |
| LEAP-Seq percent confirming: | 25.0 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGTTCACTCTCATGTGCCC |
| Suggested primer 2: | CACATACGGTTCCTGCCCTT |