| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.052805 |
| Chromosome: | chromosome 3 |
| Location: | 4546070 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g176150 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTCATGTCGCCCGCCCCCGCTCCCACCGCCTCCAACACCACCACCGCCG |
| Internal bar code: | TAACTATCGAGACATATGTTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3180 |
| LEAP-Seq percent confirming: | 90.4762 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCAATCACAGGCGCTCTAA |
| Suggested primer 2: | AGCACGACTTCTTCTTCCGG |