| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.052829 |
| Chromosome: | chromosome 16 |
| Location: | 7708023 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g686398 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAACAGGTGGCGGCGGCGCACGTGCGCGACGCGCCGCCGCCACCCGCCG |
| Internal bar code: | GCTGTATTGGGATTAGACCATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1756 |
| LEAP-Seq percent confirming: | 15.625 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTAAGGTGGCTGTAGCAC |
| Suggested primer 2: | TGCCTCAGCCAATTGTTTGC |