| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.052831 |
| Chromosome: | chromosome 2 |
| Location: | 4250708 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g104550 | Paf1 complex subunit CDC73; (1 of 1) K15175 - parafibromin (CDC73) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGCCGCCCGCCAAGGACAGTCCGGACTGGGAGCGGGTGGCGGCGGTGGT |
| Internal bar code: | CCGTTCTGAGTCCACCATCTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 228 |
| LEAP-Seq percent confirming: | 83.3333 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCTTCTCGTCGCTGAAGTG |
| Suggested primer 2: | GACGTCGCTCATCAACATGC |