| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.052837 |
| Chromosome: | chromosome 17 |
| Location: | 526355 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g699350 | SMM51 | (1 of 13) PF12847 - Methyltransferase domain (Methyltransf_18); S-adenosyl-L-methionine-dependent methyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTTTCGTGCAGTCTGGGGCGGCAAGGGCCAAGCGTACCTGGTCGCGGCG |
| Internal bar code: | AAAGCTCCCTCTTGACCTAAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2466 |
| LEAP-Seq percent confirming: | 90.9091 |
| LEAP-Seq n confirming: | 50 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 55 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCCTTGACTGCCTGTACA |
| Suggested primer 2: | GGGGCTGCTGCTAAGTAACA |