Insertion junction: CLIP2.052854_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.052854
Chromosome:chromosome 1
Location:2774058
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g016950 (1 of 1) 2.5.1.58//2.5.1.59 - Protein farnesyltransferase / FTase // Protein geranylgeranyltransferase type I / Type I protein geranyl-geranyltransferase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGGCCTTAGCCACAGGCGTGCTGCGTTCAGCATGATGGTGCAAGGGCCA
Internal bar code:TCTACCGATTAGGGATCATACT

Confirmation - LEAP-Seq

LEAP-Seq distance:2775
LEAP-Seq percent confirming:53.4483
LEAP-Seq n confirming:31
LEAP-Seq n nonconfirming:27
LEAP-Seq n unique pos:58

Suggested primers for confirmation by PCR

Suggested primer 1:CTCATGCGGTATGCTTGCAC
Suggested primer 2:ATTTAGGAGGCGGGGGTTTG