| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.052854 |
| Chromosome: | chromosome 1 |
| Location: | 2774058 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g016950 | (1 of 1) 2.5.1.58//2.5.1.59 - Protein farnesyltransferase / FTase // Protein geranylgeranyltransferase type I / Type I protein geranyl-geranyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACATCGTTAAATCCGCGCTGGCGGTGGAGCCAGTACGGTGTGCGCATGG |
| Internal bar code: | TGGTTCCCAGTTGTAGTAACCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 837 |
| LEAP-Seq percent confirming: | 65.2174 |
| LEAP-Seq n confirming: | 45 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 69 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTTAGGAGGCGGGGGTTTG |
| Suggested primer 2: | CTCATGCGGTATGCTTGCAC |