| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.052861 |
| Chromosome: | chromosome 9 |
| Location: | 2728547 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g391500 | ATG9 | (1 of 1) K17907 - autophagy-related protein 9 (ATG9); Autophagy related | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGTTTGGGCCCGAGCGCATCGCGCCAAACATCCTTTGCAACCCCCCACC |
| Internal bar code: | TCAATCTGGATGCGGCTCTAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 514 |
| LEAP-Seq percent confirming: | 59.0909 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACTTGTTGTCGGTGGGACT |
| Suggested primer 2: | TCGAGGCCGTTACTGTCAAC |