Insertion junction: CLIP2.052868_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.052868
Chromosome:chromosome 15
Location:1256702
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre15.g801725 NCL64 Nuclear Control of chloroplast-Like 64; (1 of 39) PF08373 - RAP domain (RAP) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCGCTAGACAGGACGCCTAGCCTAACCTCTCACCTTGCCACACAGGTTG
Internal bar code:GGTAGGCATTCGTTTCGTTCAA

Confirmation - LEAP-Seq

LEAP-Seq distance:4370
LEAP-Seq percent confirming:31.4607
LEAP-Seq n confirming:28
LEAP-Seq n nonconfirming:61
LEAP-Seq n unique pos:89

Suggested primers for confirmation by PCR

Suggested primer 1:AATGTTACAGTTCGCACGCG
Suggested primer 2:AGGACCTCTAGAGCCCACAG