| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.052891 |
| Chromosome: | chromosome 5 |
| Location: | 2604950 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g239800 | ARS14 | Arylsulfatase; (1 of 19) 3.1.6.1 - Arylsulfatase / Sulfatase | outside_mRNA |
| Cre05.g239850 | PHO4,PHOD | Alkaline phosphatase; (1 of 1) PTHR22953//PTHR22953:SF28 - ACID PHOSPHATASE RELATED // SUBFAMILY NOT NAMED | 5'UTR/CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCGACGAGCTCAGTTGCTCGTTGTGGGCTCGCATATTGTGCGTTGTTG |
| Internal bar code: | GAAGGATCGAGGATCTGTATCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1748 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 28 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCCCTCCTGTCGATGAAAC |
| Suggested primer 2: | TGGAAAAAGTGTGGGCTGGT |