| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.052903 |
| Chromosome: | chromosome 12 |
| Location: | 7356989 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g557800 | (1 of 1) K06875 - programmed cell death protein 5 (PDCD5, TFAR19) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCCCCTTCCACCTACCGCTTGGCCTCCTCCTGTGCCTCAATATCCTCC |
| Internal bar code: | AGTAACGTGACTATTAAGTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3157 |
| LEAP-Seq percent confirming: | 93.8144 |
| LEAP-Seq n confirming: | 91 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 97 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCTTCAACTTCGGTGGTCG |
| Suggested primer 2: | GAGTAAGGAGGGAGGGAGCA |