| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.052910 |
| Chromosome: | chromosome 12 |
| Location: | 237437 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g485050 | CAH6 | Carbonic Anhydrase 6; (1 of 3) PTHR11002//PTHR11002:SF17 - CARBONIC ANHYDRASE // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCTCACGGATAGGGCATCGCCTGAGCAGGTGCTGCAGAACCTGCTGGAC |
| Internal bar code: | TTGCGTCAGCTGAATGCCGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1586 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGACGTTCTCCTTCACCACG |
| Suggested primer 2: | TCATTTTTGTGAAGGCCGCG |