| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.052915 |
| Chromosome: | chromosome 13 |
| Location: | 3353488 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g586500 | KIN17-2,KIN17B | (1 of 32) 3.6.4.4 - Plus-end-directed kinesin ATPase / Kinesin; Kinesin motor protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTCTTTATTGACATGATCGCCTCGTCAAAGCTCAGGCGCCGCGTCAAGC |
| Internal bar code: | TAATCTTGCTTTAACATAAGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1116 |
| LEAP-Seq percent confirming: | 87.5 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTTGTCGCGATTGCAAAA |
| Suggested primer 2: | CATCGTCCTCTTCCCCATCG |