Insertion junction: CLIP2.052924_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.052924
Chromosome:chromosome 3
Location:4314514
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g174350 PWR2 (1 of 14) PF04720 - PDDEXK-like family of unknown function (PDDEXK_6); PWR motif protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATCCGACGTCAGCACAGGCGCACGGAGGCACAGACAGGGAATGAGTAGC
Internal bar code:TTTTGCACACGGGGTGATCATC

Confirmation - LEAP-Seq

LEAP-Seq distance:3804
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:46
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:46

Suggested primers for confirmation by PCR

Suggested primer 1:TTCGCAAACTTTTCGGCAGG
Suggested primer 2:GCATCAACGATGACGACGTG