| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.052927 |
| Chromosome: | chromosome 16 |
| Location: | 1214544 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g650750 | FAP268 | (1 of 18) PTHR10891 - EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN; Flagellar Associated Protein 268 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAGCGTGGCCACCTGGGTGCCAGGCAGGCAGGGGGCGAGTGGGTGGGAG |
| Internal bar code: | GGGGGTAGAGCGCCAATTCACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1272 |
| LEAP-Seq percent confirming: | 61.7647 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCAGCGCTCCTAAGTGATA |
| Suggested primer 2: | ACAATGGACTTTGCACGCAC |