Insertion junction: CLIP2.052929_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.052929
Chromosome:chromosome 5
Location:2754661
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre05.g240700 FAP378 Flagellar Associated Protein 378; (1 of 1) PF00530//PF05572 - Scavenger receptor cysteine-rich domain (SRCR) // Pregnancy-associated plasma protein-A (Peptidase_M43) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCACTTTGTGAACCCGGTGCCGCACCGAGAAATAAATTGTTAACGAGCAG
Internal bar code:GCAATTCTTTCAAGACCCAAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:525
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CTCCTGATACCGTACGTGGC
Suggested primer 2:ATAGATCCCCTGCGCATGTG