| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.052934 |
| Chromosome: | chromosome 4 |
| Location: | 2786937 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g223800 | (1 of 1) K01724 - 4a-hydroxytetrahydrobiopterin dehydratase [EC:4.2.1.96] (PCBD, phhB) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCCCAAAAGCATGCTGACAAGGCTGCCGGTCGCGCTCTCGAGGCTGCCT |
| Internal bar code: | CGGGGTACTGGTCTTGATAACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3925 |
| LEAP-Seq percent confirming: | 99.2366 |
| LEAP-Seq n confirming: | 130 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 131 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACAAGCCATCGTGCCCATT |
| Suggested primer 2: | ACTCTGCACAACACGAGAGG |