Insertion junction: CLIP2.052964_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.052964
Chromosome:chromosome 12
Location:702936
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g492850 POC19 Proteome of centriole protein 19; (1 of 1) PF10595 - Uncharacterised protein family UPF0564 (UPF0564) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGGTGGGATACTAAATGCTTGCTGCTCTTCATTTCCTGTATTTGATCTG
Internal bar code:ATGATTTGCGCATGATAGGCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:2219
LEAP-Seq percent confirming:62.1622
LEAP-Seq n confirming:23
LEAP-Seq n nonconfirming:14
LEAP-Seq n unique pos:37

Suggested primers for confirmation by PCR

Suggested primer 1:ACACACAGGTTGCAAGGTCA
Suggested primer 2:CGGATCCCGTGCATTTCAAC