| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.052981 |
| Chromosome: | chromosome 7 |
| Location: | 4523179 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g343800 | (1 of 18) PF12937 - F-box-like (F-box-like) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCAGCTCCTGACCCGTGCCCATCCCAGTCGACTCGTAGTCCCTGACGCT |
| Internal bar code: | GGCGGGACTTGCGCTGTGGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2232 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGACCGCGGATGTTGTCAAT |
| Suggested primer 2: | TCCTTGGGGATCTCCTCCAG |