| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.052991 |
| Chromosome: | chromosome 7 |
| Location: | 5443544 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g350850 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGAAGAGGTCAAAGTTAACCGGCACGTCGGCCAGGCTGCCGTTCGAGGG |
| Internal bar code: | GCTTTAGGTATGTGGGTAAGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 781 |
| LEAP-Seq percent confirming: | 30.9524 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGACAAAGATGCTGGAGGCC |
| Suggested primer 2: | GCCAGTTAAGACATGCGCAG |