| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.052996 |
| Chromosome: | chromosome 7 |
| Location: | 2281858 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g327750 | TRP3 | Transient receptor potential ion channel protein; (1 of 6) PTHR31942//PTHR31942:SF1 - FAMILY NOT NAMED // MLO-LIKE PROTEIN 1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCACACCGTGCCTCCACACCTACCTACATTCCACTCTCAAACCCACCCTC |
| Internal bar code: | ACAAGAGTATCGAACTGCTTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3329 |
| LEAP-Seq percent confirming: | 82.1429 |
| LEAP-Seq n confirming: | 46 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 56 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAAGATACGCAAGAGCACG |
| Suggested primer 2: | GGAGGTGTTCATCCGGTTGT |