Insertion junction: CLIP2.053001_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.053001
Chromosome:chromosome 17
Location:3830468
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g727335 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCATGACTGCCCTCCCCACCCCGCAGTCCAAGGTTGTCCACGCTTCGTTC
Internal bar code:GCGTGACAACGCCCATGTTATC

Confirmation - LEAP-Seq

LEAP-Seq distance:3307
LEAP-Seq percent confirming:98.2456
LEAP-Seq n confirming:56
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:57

Suggested primers for confirmation by PCR

Suggested primer 1:CGCCCTTCCAGACGTCTATC
Suggested primer 2:TCGTCGCACTTCAGCTTTCT