Insertion junction: CLIP2.053004_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.053004
Chromosome:chromosome 15
Location:5651800
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre15.g801853 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACGAATGCAACCCCTCCTACACAACACACACACACACACACACACACAC
Internal bar code:CCACCTGGTATGGGAAGAACGT

Confirmation - LEAP-Seq

LEAP-Seq distance:859
LEAP-Seq percent confirming:41.6667
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:GTGTGTGTGTGTGTGTGTGG
Suggested primer 2:CACGGGCTTTCGTTTCGTTT