| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.053004 |
| Chromosome: | chromosome 15 |
| Location: | 5651809 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g801853 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGAGAAGGTGTAGCCCGGAACAGTAGTACAGCAGGCCCAAGGGGGAGG |
| Internal bar code: | CCACCTGGTATGGGAAGAACGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 177 |
| LEAP-Seq percent confirming: | 44.4444 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACGGGCTTTCGTTTCGTTT |
| Suggested primer 2: | GTGTGTGTGTGTGTGTGTGG |