| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.053017 |
| Chromosome: | chromosome 7 |
| Location: | 5294436 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g349850 | (1 of 1) IPR000104//IPR001680//IPR017986//IPR020472 - Antifreeze protein, type I // WD40 repeat // WD40-repeat-containing domain // G-protein beta WD-40 repeat | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATTCGTTGCGGACCGGAAGACGAGCGGGCACGGCCCGGACTTGCAGCGA |
| Internal bar code: | TTCGGGGCTGAACGCTCGCTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2209 |
| LEAP-Seq percent confirming: | 94.7368 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATCTCTTCAAGCGCCATG |
| Suggested primer 2: | CGGGGCTTGTTTTGTGTCAG |