Insertion junction: CLIP2.053017_3

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.053017
Chromosome:chromosome 17
Location:5735074
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g740800 (1 of 5) PF06742//PF06863 - Protein of unknown function (DUF1214) (DUF1214) // Protein of unknown function (DUF1254) (DUF1254) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGAACGACTGCGCCGCATATGCCGCTGCTGGCACACACACGCAGCCGGTA
Internal bar code:TTCAACTCTAGTGCGAGATCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:1218
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:ACATACACACACGTCACCCC
Suggested primer 2:CTGCTCAACGTGACGAGTCT