Insertion junction: CLIP2.053037_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.053037
Chromosome:chromosome 11
Location:916323
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g467655 IMP1 Mitochondrial inner membrane signal peptidase; (1 of 1) K09647 - mitochondrial inner membrane protease subunit 1 (IMP1) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATTCCCTACGCCCCGCTGCCCGCAAGCTGCCCGCAAGCCGCACCACACCG
Internal bar code:GCTCATTGTACTTATGTCATAA

Confirmation - LEAP-Seq

LEAP-Seq distance:291
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CGCCCTTCACTTTGTGCTTC
Suggested primer 2:ACAGCGACATGACAGGAAGG