Insertion junction: CLIP2.053042_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.053042
Chromosome:chromosome 6
Location:3462941
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre06.g278107 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTTGAGTAGTTAATCCATCCACCGCTCGCGACTACTCGCCAGCTTTCCG
Internal bar code:GTGCGCTCATCTTAGACGTATG

Confirmation - LEAP-Seq

LEAP-Seq distance:414
LEAP-Seq percent confirming:66.6667
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:ACACGGGTCATGTGGAAGTC
Suggested primer 2:GTCCAAGCTACCCCAACCTC