| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.053053 |
| Chromosome: | chromosome 16 |
| Location: | 421157 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g693450 | ISG-C1 | (1 of 32) IPR029062 - Class I glutamine amidotransferase-like | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACTTGCGGAGGCTTCCAGGAAAGCGTTCCTTGTTGAGGGCCGTGCGGCA |
| Internal bar code: | ATGGCTATATGTTACTTAACTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 622 |
| LEAP-Seq percent confirming: | 74.2857 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGAGCATGGAGATGAGGGA |
| Suggested primer 2: | GACGATCAGGGCAGATCAGG |