Insertion junction: CLIP2.053100_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.053100
Chromosome:chromosome 2
Location:922418
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g079100 XPA1 DNA repair protein; (1 of 1) K10847 - DNA-repair protein complementing XP-A cells (XPA) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTAGGAAAGGACCCAAGGACAAACGGCGGCAGCCGAGAGGGCGCAGGCAG
Internal bar code:TTGTGTCATCATCCGCGGTAAT

Confirmation - LEAP-Seq

LEAP-Seq distance:173
LEAP-Seq percent confirming:50.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GGTGCACAGCAGTTGAAAGG
Suggested primer 2:CAAAGCGAGTTGGTGTGTGG